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Detection Image Detection method details

Name:

 QT-EVE-BN-013

Description:

 Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event LBFLFK locus2 (EU-RL GMFF GMOMETHODS database as of October 12, 2022)

Comment:

 Target is the 5' integration border region (IBR) between the insert of oilseed rape event LBFLFK Locus2 and the oilseed rape host genome.

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

 QT-TAX-BN-001

Type:

 event-specific

Target GMO name:

 LBFLFK

  • Oligonucleotides:

  • Forward Primer

  • Name:

    rightFST_ev1_loc2-f4

  • Sequence:

    GATTGGTAATATGTAAATAACGGGATCC

  • Size:

    28

  • Reverse Primer

  • Name:

    rightFST_ev1_loc2-r1

  • Sequence:

    GCGAATTTGGCCTGTAGACC

  • Size:

    20

  • Probe

  • Name:

    rightFST_ev1_loc2-s1

  • Sequence:

    CATCACACCAAAAGTTAGGCCCGAA

  • Size:

    25

  • Amplicon:

  • Sequence:

    gattggtaat atgtaaataa cgggatccnn nnnnnnnnnn nnnnnnnnnc atcacaccaa aagttaggcc cgaannnnnn nnnnnnnnnn nnnnnnnnnn nnnggtctac aggccaaatt cgc

  • Size:

    123

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-EVE-BN-013Link to the document url. EU reference method protocol