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Detection Image Detection method details

Name:

 QL-CON-307-F/652-R

Description:

 Qualitative real-time PCR (TaqMan) method for detection of the junction between the CaMV 35S promoter and the hpt gene (Reiting et al., 2013).

Comment:

 Target is the junction region between the Cauliflower mosaic virus (CaMV) 35S promoter (P-35S) and the hygromycin phophotransferase II (hpt) gene.

The sequence of the probe only corresponds to the sequence displayed in figure 1 of the source publication (wrong sequence in table 1). The correctness of the probe sequence as displayed here was confirmed by the author of the publication (2023).

Validation:

 in-house validation

Standardisation:

 none

Related Methods:

Type:

 construct-specific

Target GMO names:
KMD1, Kefeng6

  • Oligonucleotides:

  • Forward Primer

  • Name:

    307-F

  • Sequence:

    GACGTAAGGGATGACGCACAA

  • Size:

    21

  • Reverse Primer

  • Name:

    652-R

  • Sequence:

    TGAGTTCAGGCTTTTTCATATCTCAT

  • Size:

    26

  • Probe

  • Name:

    307-T

  • Sequence:

    CCCACTATCCTTCGCAAGACCCTTCC

  • Size:

    26

  • Amplicon:

  • Sequence:

    gacgtaagggatgacgcacaatcccactatccttcgcaagacccttcctctatataaggaagttcatttcatttggagaggacacgctgaaatcaccagtctctctctacaaatctatctctctcgagctttcgcagatccggggggcaatgagatatgaaaaagcctgaactca

  • Size:

    175

Documents

Citation Type Local copy
Reiting R, Grohmann L, Moris G, Maede D (2013) Detection and characterization of an unknown rice event in Basmati rice products. Eur. Food Res. Technol. 236:715-723Link to the document url. peer-reviewed article