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Detection Image Detection method details

Name:

 QL-CON-00-010

Description:

 Qualitative real-time PCR (TaqMan) method for detection of a construct present in flax event FP967 (ISO 21569-2)

Comment:

 Target is the junction region between the nopaline synthase terminator (T-nos) from Agrobacterium tumefaciens and the dihydrofolate reductase (dhfr) gene from Escherichia coli

Validation:

 ring trial validation

Standardisation:

 ISO standard

Related Methods:

 QL-TAX-LU-001

Type:

 construct-specific

Target GMO names:
FP967

  • Oligonucleotides:

  • Forward Primer

  • Name:

    NOST-Spec FW

  • Sequence:

    AGCGCGCAAACTAGGATAAA

  • Size:

    20

  • Reverse Primer

  • Name:

    NOST-Spec RV

  • Sequence:

    ACCTTCCGGCTCGATGTCTA

  • Size:

    20

  • Probe

  • Name:

    NOST-Spec Probe

  • Sequence:

    CGCGCGCGGTGTCATCTATG

  • Size:

    20

  • Amplicon:

  • Sequence:

    agcgcgcaaactaggataaannnncgcgcgcggtgtcatctatgnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntagacatcgagccggaaggt

  • Size:

    105

Documents

Citation Type Local copy
Horizontal methods for molecular biomarker analysis - Methods of analysis for the detection of genetically modified organisms and derived products - Part 2: Construct-specific real-time PCR method for detection of event FP967 in linseed and linseed products (ISO/TS 21569-2)Link to the document url. unknown
EU-RL GMFF GMOMETHODS database; QL-CON-00-010Link to the document url. EU reference method protocol