Help icon

Detection Image Detection method details

Name:

 QT-TAX-SPS primer f/SPS primer r

Description:

 Quantitative real-time PCR (TaqMan) method for detection of the rice sucrose phosphate synthase (SPS) gene (ISO21570 Amd1)

Comment:

 Target is the endogenous sucrose phosphate synthase (SPS) gene from Oryza sativa

Validation:

 ring trial validation

Standardisation:

 ISO standard

Related Methods:

Type:

 taxon-specific

Target Species name:

 Oryza sativa

  • Oligonucleotides:

  • Forward Primer

  • Name:

    SPS primer f

  • Sequence:

    TTGCGCCTGAACGGATAT

  • Size:

    18

  • Reverse Primer

  • Name:

    SPS primer r

  • Sequence:

    CGGTTGATCTTTTCGGGATG

  • Size:

    20

  • Probe

  • Name:

    SPS probe

  • Sequence:

    TCCGAGCCGTCCGTGCGTC

  • Size:

    19

  • Amplicon:

  • Sequence:

    ttgcgcctgaacggatatctttcagtttgtaaccaccggatgacgcacggacggctcggatcatcccgaaaagatcaaccg

  • Size:

    81

Documents

Citation Type Local copy
Yang L., Yang Y., Jin W., Zhang X., Li X., Wu Y., Li J., Li L. (2018) Development and interlaboratories validation of event-specific quantitative real-time PCR method for genetically modified rice G6H1 event. Journal of Agricultural and Food Chemistry, 66(30),8179-8186Link to the document url. peer-reviewed article
Ding J, Jia, J, Yang L, Wen H, Zhang C, Liu W, Zhang D (2004) Validation of a rice specific gene, sucrose phosphate synthase, used as the endogenous reference gene for qualitative and real-time quantitative PCR detection of transgenes. J Agric Food Chem 5Link to the document url.