Help icon

Detection Image Detection method details

Name:

 QT-EVE-ZM-005

Description:

 Quantitative real-time PCR (TaqMan) method for detection of maize event MON87460 (EU-RL GMFF GMOMETHODS database as of July 21, 2014)

Comment:

 Target is the 5' integration border region (IBR) between the insert of maize event MON87460 and the maize host genome

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

 QT-TAX-ZM-002

Type:

 event-specific

Target GMO name:

 MON87460

  • Oligonucleotides:

  • Forward Primer

  • Name:

    MON 87460 primer 1

  • Sequence:

    CACGTTGAAGGAAAATGGATTG

  • Size:

    22

  • Reverse Primer

  • Name:

    MON 87460 Primer 2

  • Sequence:

    TCGCGATCCTCCTCAAAGAC

  • Size:

    20

  • Probe

  • Name:

    MON 87460 Probe

  • Sequence:

    AGGGAGTATGTAGATAAATTTTCAAAGCGTTAGACGGC

  • Size:

    38

  • Amplicon:

  • Sequence:

    cacgttgaaggaaaatggattgnagggagtatgtagataaattttcaaagcgttagacggcngtctttgaggaggatcgcga

  • Size:

    82

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-EVE-ZM-005Link to the document url. EU reference method protocol