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Detection Image Detection method details

Name:

 QT-ELE-00-003

Description:

 Quantitative real-time PCR (TaqMan) method for detection of synthetic cryIA(b) gene (ISO/FDIS 21570)

Comment:

 Target is the synthetic cryIA(b) gene

Validation:

 ring trial validation

Standardisation:

 ISO standard

Related Methods:

Type:

 element-specific

Target DNA element:
CS-cry1Ab-BACTU

  • Oligonucleotides:

  • Forward Primer

  • Name:

    CRY2-F

  • Sequence:

    CCCATCGACATCAGCCTGAGC

  • Size:

    21

  • Reverse Primer

  • Name:

    CRY2-R

  • Sequence:

    CAGGAAGGCGTCCCACTGGC

  • Size:

    20

  • Probe

  • Name:

    BTSYN

  • Sequence:

    ATGTCCACCAGGCCCAGCACG

  • Size:

    21

  • Amplicon:

  • Sequence:

    cccatcgacatcagcctgagcnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnngccagtgggacgccttcctg

  • Size:

    129

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-ELE-00-003Link to the document url. EU reference method protocol
Foodstuffs - Methods of analysis for the detection of genetically modified organisms and derived products - Quantitative nucleic acid based methods ISO 21570:1-103 (2005)Link to the document url. other document
Grisolia, C. K., Oliveira, R., Domingues, I., Oliveira-Filho, E. C., Monerat, R. G., & Soares, A. M. V. M. (2009). Genotoxic evaluation of different delta-endotoxins from Bacillus thuringiensis on zebrafish adults and development in early life stages. Mutation Research-Genetic Toxicology and Environmental Mutagenesis, 672(2), 119-123.Link to the document url. peer-reviewed article