Detection method details
Name: |
QL-TAX-CaMVF/CaMVR |
Description: |
Qualitative real-time PCR (TaqMan) method for detection of ORF IV of the coat protein of CaMV virus (Cancar et al., 2005) |
Comment: |
target is the ORF IV of Cauliflower Mosaic Virus (CaMV) |
Validation: |
in-house validation |
Standardisation: |
none |
Related Methods: |
Type: |
taxon-specific |
Target Species name: |
Cauliflower mosaic virus |
-
Oligonucleotides:
Forward Primer
Name:
CaMVFSequence:
GGCCATTACGCCAACGAATSize:
19
Reverse Primer
Name:
CaMVRSequence:
ATGGGCTGGAGACCCAATTTTSize:
21
Probe
Name:
CaMV-MGBSequence:
TTCTCCGAGCTTTGTCSize:
16
-
Amplicon:
Sequence:
GGCCATTACGCCAACGAATGTCCTAATCGACAAAGCTCGGAGAAGGCTCACATCCTTCAACAAGCAGAGAAAATTGGGTCTCCAGCCCATSize:
90
Documents
Citation | Type | Local copy |
---|---|---|
Cankar K, Ravnikar M, Žel J, Gruden K, Toplak N (2005) Real-time polymerase chain reaction detection of Cauliflower mosaic virus to complement the 35S screening assay for genetically modified organisms. J AOAC Int 88:814-822 |