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Detection Image Detection method details

Name:

 QL-TAX-AT-001

Description:

 Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase (nos) gene (ISO/TS 21569-7, 2022).

Comment:

 Target is the nopaline synthase gene (nos) from Agrobacterium tumefaciens (indicating the presence of Ti-plasmid from phytopathogenic Agrobacterium strains).

Validation:

 ring trial validation

Standardisation:

 ISO standard

Related Methods:

Type:

 element-specific

Target DNA element:
CS-nos-RHIRD

  • Oligonucleotides:

  • Forward Primer

  • Name:

    At-nop-f2

  • Sequence:

    CCAGCCRTSTACTGATTATTGTMAC

  • Size:

    25

  • Reverse Primer

  • Name:

    At-nop-r2

  • Sequence:

    TGCGAGTTCRCCGTTGAAG

  • Size:

    19

  • Probe

  • Name:

    At-nop-s1

  • Sequence:

    CCGTGCGGACGTTCACGACAG

  • Size:

    21

  • Amplicon:

  • Sequence:

    tgcgagttcrccgttgaagttggcgagttcgttgacgaagctgtcgtgaacgtccgcacgggtkacaataatcagtasayggctgg

  • Size:

    86

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QL-TAX-AT-001Link to the document url. EU reference method protocol