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Detection Image Detection method details

Name:

 QL-EVE-ZM-002

Description:

 Qualitative conventional PCR method for detection of maize event Bt10 (verified by the EU-RL GMFF in the context of Commission Decision 317/2005/EC)

Comment:

 A target is not defined. However, this method specifically detects Bt10 maize an can discriminate between Bt10 and other maize events, also between Bt10 and Bt11.

Validation:

 in-house validation

Standardisation:

 none

Related Methods:

Type:

 event-specific

Target GMO name:

 Bt10 maize

  • Oligonucleotides:

  • Forward Primer

  • Name:

    JSF5

  • Sequence:

    CACACAGGAGATTATTATAGGGTTACTCA

  • Size:

    29

  • Reverse Primer

  • Name:

    JSR5

  • Sequence:

    ACACGGAAATGTTGAATACTCATACTCT

  • Size:

    28

  • Amplicon:

  • Sequence:

    cacacaggagattattatagggttactcannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnagagtatgagtattcaacatttccgtgt

  • Size:

    117

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QL-EVE-ZM-002Link to the document url. EU reference method protocol