Detection method details
Name: |
QT-EVE-BN-008 |
Description: |
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event Topas 19/2 (EU-RL GMFF GMOMETHODS database as of July 21, 2014) |
Comment: |
Target is the 3' integration border region (IBR) between the insert of oilseed rape event Topas 19/2 and the oilseed rape host genome |
Validation: |
in-house validation |
Standardisation: |
EU reference method |
Related Methods: |
QT-TAX-BN-012 |
Type: |
event-specific |
Target GMO name: |
Topas 19/2 |
-
Oligonucleotides:
Forward Primer
Name:
MDB685Sequence:
GTTGCGGTTCTGTCAGTTCCSize:
20
Reverse Primer
Name:
KVM180Sequence:
CGACCGGCGCTGATATATGASize:
20
Probe
Name:
TM029Sequence:
TCCCGCGTCATCGGCGGSize:
17
-
Amplicon:
Sequence:
gttgcggttctgtcagttccnnnnnnnnnnnnnnnnntcccgcgtcatcggcggnnnnnnnnnnnnnnnnnnnnntcatatatcagcgccggtcgSize:
95
Documents
Citation | Type | Local copy |
---|---|---|
EU-RL GMFF GMOMETHODS database; QT-EVE-BN-008 | EU reference method protocol |