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Detection Image Detection method details

Name:

 QT-TAX-zSSIIb-3F/zSSIIb-4R

Description:

 Quantitative real-time PCR (TaqMan) method for detection of maize starch synthase IIb gene (Yang et al., 2005)

Comment:

 Target is the endogenous starch synthase IIb (zSSIIb) gene from Zea mays.

Validation:

 unknown

Standardisation:

 unknown

Related Methods:

Type:

 taxon-specific

Target Species name:

 Zea mays

  • Oligonucleotides:

  • Forward Primer

  • Name:

    zSSIIb-3F

  • Sequence:

    CGGTGGATGCTAAGGCTGATG

  • Size:

    21

  • Reverse Primer

  • Name:

    zSSIIb-4R

  • Sequence:

    AAAGGGCCAGGTTCATTATCCTC

  • Size:

    23

  • Probe

  • Name:

    zSSIIb-P

  • Sequence:

    TAAGGAGCACTCGCCGCCGCATCTG

  • Size:

    25

  • Amplicon:

  • Sequence:

    cggtggatgctaaggctgatgcagctccggctacagatgcggcggcgagtgctccttatgacagggaggataatgaacctggcccttt

  • Size:

    88

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