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Detection Image Detection method details

Name:

 QL-ELE-00-018

Description:

 Qualitative real-time PCR (SYBRGreen) method for detection of T-nos-RHIRD (Barbau-Piednoir et al., 2014)

Comment:

 Target is the T-nos-RHIRD from Agrobacterium tumefaciens

Validation:

 ring trial validation

Standardisation:

 unknown

Related Methods:

 QL-TAX-BN-003, QL-TAX-GM-001, QL-TAX-ZM-002

Type:

 element-specific

Target DNA element:
T-nos-RHIRD

  • Oligonucleotides:

  • Forward Primer

  • Name:

    tNOS_NN_Fwd

  • Sequence:

    GATTAGAGTCCCGCAATTATACATTTAA

  • Size:

    28

  • Reverse Primer

  • Name:

    tNOS D REV

  • Sequence:

    TTATCCTAGKTTGCGCGCTATATTT

  • Size:

    25

  • Amplicon:

  • Sequence:

    gattagagtcccgcaattatacatttaannnnnnnnnnnnnnnnaaatatagcgcgcaamctaggataa

  • Size:

    69

Documents

Citation Type Local copy
Barbau-Piednoir, E., Stragier, P., Roosens, N., Mazzara, M., Savini, C., Van den Eede, G., Van den Bulcke, M. (2014). Inter-laboratory testing of GMO detection by combinatory SYBR®Green PCR screening (CoSYPS). Food Analytical Methods. 7, 1719-1728.Link to the document url. peer-reviewed article
EU-RL GMFF GMOMETHODS database; QL-ELE-00-018Link to the document url. EU reference method protocol