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Detection Image Detection method details

Name:

 QL-CON-00-007

Description:

 Qualitative real-time PCR (TaqMan) method for detection of rice event Bt63 (ISO 21569-Amd)

Comment:

 Target is the junction region between the CS-cry1Ab_Ac-SYNTH fusion gene and the DNA spacer sequences linking the fusion gene to T-nos-RHIRD (nopaline synthase terminator)

Validation:

 ring trial validation

Standardisation:

 ISO standard

Related Methods:

Type:

 construct-specific

Target GMO names:
Huahui No.1

  • Oligonucleotides:

  • Forward Primer

  • Name:

    T51F

  • Sequence:

    GACTGCTGGAGTGATTATCGACAGA

  • Size:

    25

  • Reverse Primer

  • Name:

    T51R

  • Sequence:

    AGCTCGGTACCTCGACTTATTCAG

  • Size:

    24

  • Probe

  • Name:

    T51p

  • Sequence:

    TCGAGTTCATTCCAGTTACTGCAACACTCGAG

  • Size:

    32

  • Amplicon:

  • Sequence:

    gactgctggagtgattatcgacagannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnctgaataagtcgaggtaccgagct

  • Size:

    83

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QL-CON-00-007Link to the document url. EU reference method protocol
Grohmann L., Maede D.; Detection of genetically modified rice: collaborative validation study of a construct-specific real-time PCR method for detection of transgenic Bt rice Eur. Food Res. Technol. 228:497-500 (2009)Link to the document url. peer-reviewed article