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Detection Image Detection method details

Name:

 QL-TAX-BN-004

Description:

 Qualitative conventional PCR method for detection of oilseed rape high mobility group protein I/Y gene (Pan et al., 2007).

Comment:

 Target is the high mobility group protein I/Y (HMG IY) gene from rapeseed (Brassica napus)

Validation:

 ring trial validation

Standardisation:

 unknown

Related Methods:

Type:

 taxon-specific

Target Species name:

 Brassica napus

  • Oligonucleotides:

  • Forward Primer

  • Name:

    HMG-1

  • Sequence:

    GGTCGTCCTCCTAAGGCGAAAG

  • Size:

    22

  • Reverse Primer

  • Name:

    HMG-2

  • Sequence:

    GCAACCAACAGGCACCATC

  • Size:

    19

  • Amplicon:

  • Sequence:

    ggtcgtcctcctaaggcgaaagnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnngatggtgcctgttggttgc

  • Size:

    219

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QL-TAX-BN-004Link to the document url. EU reference method protocol
Pan L., Zhang S., Yang L., Broll H., Tian F., Zhang D.; Interlaboratory trial validation of an event-specific qualitative polymerase chain reaction-based detection method for genetically modified RT73 rapeseed J AOAC Int 90:1639-1646 (2007)Link to the document url. peer-reviewed article