Detection method details
Name: |
QL-ELE-epsps2 1-5'/epsps2 1-3' |
Description: |
Qualitative real-time PCR (TaqMan) method for detection of the CP4 epsps gene (Mano et al., 2009) |
Comment: |
target is the CP4 epsps gene from bacterium Agrobacterium tumefaciens |
Validation: |
in-house validation |
Standardisation: |
none |
Related Methods: |
Type: |
element-specific |
Target DNA element: |
CS-CP4epsps-RHIRD
|
-
Oligonucleotides:
Forward Primer
Name:
epsps2 1-5'Sequence:
GTCTCGTTTCTGAAAACCCTGTSize:
22
Reverse Primer
Name:
epsps2 1-3'Sequence:
TTAGTGTCGGAGAGTTCGATCTTAGSize:
25
Probe
Name:
epsps2-1-TaqSequence:
TGATCGCTACTAGCTTCCCAGAGTTCATGGSize:
29
-
Amplicon:
Sequence:
GTCTCGTTTCTGAAAACCCTGTTACTGTTGATGATGCTACTATGATCGCTACTAGCTTCCCAGAGTTCATGGATTTGATGGCTGGTCTTGGAGCTAAGATCGAACTCTCCGACACTAASize:
118