Detection method details
Name: |
QT-EVE-ZM-023 |
Description: |
Quantitative real-time PCR (TaqMan) method for detection of maize event Bt176 (EU-RL GMFF GMOMETHODS database as of October 01, 2014). |
Comment: |
Target is the 3' integration border region (IBR) between the insert of maize event Bt176 and the maize host genome. |
Validation: |
in-house validation |
Standardisation: |
EU reference method |
Related Methods: |
QT-TAX-ZM-014 |
Type: |
event-specific |
Target GMO name: |
Bt176 |
-
Oligonucleotides:
Forward Primer
Name:
Bt176 FSequence:
GGCCGTGAACGAGCTGTTSize:
18
Reverse Primer
Name:
Bt176 RSequence:
GGGAAGAAGCCTACATGTTTTCTAASize:
25
Probe
Name:
Bt176 ProbeSequence:
AGCAACCAGATCGGCCGACACCSize:
22
-
Amplicon:
Sequence:
ggccgtgaacgagctgttnnnnnnnagcaaccagatcggccgacaccnnnnnnnnnnttagaaaacatgtaggcttcttcccSize:
82
Documents
Citation | Type | Local copy |
---|---|---|
EU-RL GMFF GMOMETHODS database; QT-EVE-ZM-023 | EU reference method protocol |