Help icon

Detection Image Detection method details

Name:

 QL-TAX-CMV-001

Description:

 Qualitative real-time PCR (TaqMan) method for detection of CaMV ORF V (ISO/TS 21569-7, 2022).

Comment:

 Target is the open reading frame V (ORF V) from Cauliflower mosaic virus (CaMV)

Validation:

 ring trial validation

Standardisation:

 ISO standard

Related Methods:

Type:

 taxon-specific

Target Species name:

 Cauliflower mosaic virus

  • Oligonucleotides:

  • Forward Primer

  • Name:

    CaMV-ORFV-fd2

  • Sequence:

    ATYAAGCCCAGYAAAAGCCC

  • Size:

    20

  • Reverse Primer

  • Name:

    CaMV-ORFV-rd2

  • Sequence:

    CTYCGCTTCTCGGCTTCRTT

  • Size:

    20

  • Probe

  • Name:

    CaMV-ORFV-P2

  • Sequence:

    CATGGCACCAGCCTTCTTGGTCAAC

  • Size:

    25

  • Amplicon:

  • Sequence:

    atyaagcccagyaaaagcccnnncatggcaccagccttcttggtcaacaaygaagccgagaagcgrag

  • Size:

    68

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QL-TAX-CMV-001Link to the document url. EU reference method protocol