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Detection Image Detection method details

Name:

QL-ELE-00-004

Description:

Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (Lipp et al., 2001)

Comment:

Target is the Cauliflower mosaic virus 35S promoter (CaMV P-35S)

Validation:

ring trial validation

Standardisation:

ISO standard

Related Methods:

Type:

element-specific

Target DNA element:

P-Cauliflower mosaic virus
  • Oligonucleotides:

  • Forward Primer

  • Name:

    35S-cf3
  • Sequence:

    CCACGTCTTCAAAGCAAGTGG
  • Size:

    21
  • Reverse Primer

  • Name:

    35S-cr4
  • Sequence:

    TCCTCTCCAAATGAAATGAACTTCC
  • Size:

    25
  • Amplicon:

  • Sequence:

    ccacgtcttcaaagcaagtggnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnggaagttcatttcatttggagagga
  • Size:

    123

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