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Detection Image Detection method details

Name:

 QT-EVE-ZM-013

Description:

 Quantitative real-time PCR (TaqMan) method for detection of maize event MIR604 (EU-RL GMFF GMOMETHODS database as of October 01, 2014)

Comment:

 Target is the 5' integration border region (IBR) between the insert of maize event MIR604 and the maize host genome

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

 QT-TAX-ZM-014

Type:

 event-specific

Target GMO name:

 MIR604

  • Oligonucleotides:

  • Forward Primer

  • Name:

    MIR604 primer F

  • Sequence:

    GCGCACGCAATTCAACAG

  • Size:

    18

  • Reverse Primer

  • Name:

    MIR604 primer R

  • Sequence:

    GGTCATAACGTGACTCCCTTAATTCT

  • Size:

    26

  • Probe

  • Name:

    MIR604 primer Probe

  • Sequence:

    AGGCGGGAAACGACAATCTGATCATG

  • Size:

    26

  • Amplicon:

  • Sequence:

    gcgcacgcaattcaacagnaggcgggaaacgacaatctgatcatgnnnnnagaattaagggagtcacgttatgacc

  • Size:

    76

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-EVE-ZM-013Link to the document url. EU reference method protocol