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Detection Image Detection method details

Name:

 QL-TAX-CaMVF/CaMVR

Description:

 Qualitative real-time PCR (TaqMan) method for detection of ORF IV of the coat protein of CaMV virus (Cancar et al., 2005)

Comment:

 target is the ORF IV of Cauliflower Mosaic Virus (CaMV)

Validation:

 in-house validation

Standardisation:

 none

Related Methods:

Type:

 taxon-specific

Target Species name:

 Cauliflower mosaic virus

  • Oligonucleotides:

  • Forward Primer

  • Name:

    CaMVF

  • Sequence:

    GGCCATTACGCCAACGAAT

  • Size:

    19

  • Reverse Primer

  • Name:

    CaMVR

  • Sequence:

    ATGGGCTGGAGACCCAATTTT

  • Size:

    21

  • Probe

  • Name:

    CaMV-MGB

  • Sequence:

    TTCTCCGAGCTTTGTC

  • Size:

    16

  • Amplicon:

  • Sequence:

    GGCCATTACGCCAACGAATGTCCTAATCGACAAAGCTCGGAGAAGGCTCACATCCTTCAACAAGCAGAGAAAATTGGGTCTCCAGCCCAT

  • Size:

    90

Documents

Citation Type Local copy
Cankar K, Ravnikar M, Žel J, Gruden K, Toplak N (2005) Real-time polymerase chain reaction detection of Cauliflower mosaic virus to complement the 35S screening assay for genetically modified organisms. J AOAC Int 88:814-822