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Detection Image Detection method details

Name:

 QL-ELE-00-017

Description:

 Qualitative real-time PCR (SYBRGreen) method for detection of P-35S CaMV (Barbau-Piednoir et al., 2014)

Comment:

 Target is the P-35S CaMV from Cauliflower mosaic virus

Validation:

 ring trial validation

Standardisation:

 unknown

Related Methods:

 QL-TAX-BN-003, QL-TAX-GM-001, QL-TAX-ZM-002

Type:

 element-specific

Target DNA element:
P-Cauliflower mosaic virus

  • Oligonucleotides:

  • Forward Primer

  • Name:

    35S_N3Fwd

  • Sequence:

    AAAGCAAGTGGATTGATGTGATA

  • Size:

    23

  • Reverse Primer

  • Name:

    35S_N3Rev

  • Sequence:

    GGGTCTTGCGAAGGATAGTG

  • Size:

    20

  • Amplicon:

  • Sequence:

    aaagcaagtggattgatgtgatannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnncactatccttcgcaagaccc

  • Size:

    75

Documents

Citation Type Local copy
Barbau-Piednoir, E., Stragier, P., Roosens, N., Mazzara, M., Savini, C., Van den Eede, G., Van den Bulcke, M. (2014). Inter-laboratory testing of GMO detection by combinatory SYBR®Green PCR screening (CoSYPS). Food Analytical Methods. 7, 1719-1728.Link to the document url. peer-reviewed article
EU-RL GMFF GMOMETHODS database; QL-ELE-00-017Link to the document url. EU reference method protocol