Detection method details
Name: |
QL-ELE-00-005 |
Description: |
Qualitative conventional PCR method for detection of Cauliflower mosaic virus 35S promoter (ISO/FDIS 21569) |
Comment: |
Target is the Cauliflower mosaic virus 35S promoter (CaMV P-35S) |
Validation: |
ring trial validation |
Standardisation: |
ISO standard |
Related Methods: |
Type: |
element-specific |
Target DNA element: |
P-Cauliflower mosaic virus
|
-
Oligonucleotides:
Forward Primer
Name:
35S-1Sequence:
GCTCCTACAAATGCCATCASize:
19
Reverse Primer
Name:
35S-2Sequence:
GATAGTGGGATTGTGCGTCASize:
20
-
Amplicon:
Sequence:
gctcctacaaatgccatcannnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntgacgcacaatcccactatcSize:
195
Documents
Citation | Type | Local copy |
---|---|---|
Foodstuffs - Methods of analysis for the detection of genetically modified organisms and derived products - Qualitative nucleic acid based methods ISO 21569:1-69 (2005) | other document | |
EU-RL GMFF GMOMETHODS database; QL-ELE-00-005 | EU reference method protocol |