Detection method details
Name: |
QL-ELE-cry1A.105 - F1/cry1A.105 - R1 |
Description: |
Qualitative real-time PCR (TaqMan) method for detection of the cry1A.105 gene (Dinon et al. 2011). |
Comment: |
Target is the synthetic cry1A.105 gene. |
Validation: |
in-house validation |
Standardisation: |
none |
Related Methods: |
Type: |
element-specific |
Target DNA element: |
CS-cry1A_105-SYNTH
|
-
Oligonucleotides:
Forward Primer
Name:
cry1A.105 - F1Sequence:
TCAGAGGTCCAGGGTTTACAGGSize:
22
Reverse Primer
Name:
cry1A.105 - R1Sequence:
GTAGTAGAGGCATAGCGGATTCTTGSize:
25
Probe
Name:
cry1A.105 - PSequence:
AGACATTCTTCGTCGCACAAGTGGAGGACCSize:
30
-
Amplicon:
Sequence:
tcagaggtccagggtttacaggaggagacattcttcgtcgcacaagtggaggaccctttgcttacactattgttaacatcaatggccaattgccccaaaggtatcgtgcaagaatccgctatgcctctactacSize:
133