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Detection Image Detection method details

Name:

 QL-CON-PGS-bar-A2/PGS-bar-B2

Description:

 Qualitative conventional PCR method for detection of the junction between P-SSU promoter and the bar gene (BVL G30.40-13).

Comment:

 Target is the junction region between the small subunit promoter (P-SSU) from Arabidopsis thaliana and the phosphinothricin acetyltransferase gene (bar) from Streptomyces hygroscopicus (BVL G30.40-13).

Validation:

 ring trial validation

Standardisation:

 national standard

Related Methods:

Type:

 construct-specific

Target GMO names:
Ms1, Ms11, Ms8, RM3-3, RM3-4, RM3-6, Rf1, Rf2, Rf3, Rf3 (Brassica juncea)

  • Oligonucleotides:

  • Forward Primer

  • Name:

    PGS-bar-A2

  • Sequence:

    GAAGTTGACCGTGCTTGTCT

  • Size:

    20

  • Reverse Primer

  • Name:

    PGS-bar-B2

  • Sequence:

    CAAGTCCACCAGGCAAGTAA

  • Size:

    20

  • Amplicon:

    unknown

Documents

Citation Type Local copy
PCR detection of the pSSUAra-bar construct for screening of genetically modified oilseed rape lines - construct-specific method. BVL Official Method Collection G 30.40-13 Online Publication (2015)Link to the document url. other document