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Detection Image Detection method details

Name:

 QT-ELE-NOS ter 2-5' /NOS ter 2-3'

Description:

 Quantitative real-time PCR (TaqMan) method for detection of T-nos (Kodama et al., 2009)

Comment:

 target is the nopaline synthase terminator (T-nos) from bacterium Agrobacterium tumefaciens. Amplicon was generated from cotton LLcotton25 (GenBank HQ233646.1)

Validation:

 ring trial validation

Standardisation:

 none

Related Methods:

Type:

 element-specific

Target DNA element:
T-nos-RHIRD

  • Oligonucleotides:

  • Forward Primer

  • Name:

    NOS ter 2-5'

  • Sequence:

    GTCTTGCGATGATTATCATATAATTTCTG

  • Size:

    29

  • Reverse Primer

  • Name:

    NOS ter 2-3'

  • Sequence:

    CGCTATATTTTGTTTTCTATCGCGT

  • Size:

    25

  • Probe

  • Name:

    NOS-Taq

  • Sequence:

    AGATGGGTTTTTATGATTAGAGTCCCGCAA

  • Size:

    30

  • Amplicon:

  • Sequence:

    GTCTTGCGATGATTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATGTAATGCATGACGTTATTTATGAGATGGGTTTTTATGATTAGAGTCCCGCAATTATACATTTAATACGCGATAGAAAACAAAATATAGCG

  • Size:

    151

Documents

Citation Type Local copy
Hideo Kuribara, Yoichiro Shindo, Takeshi Matsuoka, Ken Takubo, Satoshi Futo, Nobutaro Aoki, Takashi Hirao, Hiroshi Akiyama, Yukihiro Goda, Masatake Toyada, Akihiro Hino. Novel reference molecules for quantitation of genetically modified maize and soybean.Link to the document url.