Detection method details
Name: |
QT-EVE-GM-004 |
Description: |
Quantitative real-time PCR (TaqMan) method for detection of soybean event A2704-12 (EU-RL GMFF GMOMETHODS database as of October 02, 2014) |
Comment: |
Target is a junction region comprising an inverted repeat of CS-bla-ECOLX in soybean event A2704-12: 3' bla sequence and an inverted 5' bla sequence |
Validation: |
ring trial validation |
Standardisation: |
EU reference method |
Related Methods: |
QT-TAX-GM-009 |
Type: |
event-specific |
Target GMO name: |
A2704-12 |
-
Oligonucleotides:
Forward Primer
Name:
KVM175Sequence:
GCAAAAAAGCGGTTAGCTCCTSize:
21
Reverse Primer
Name:
SMO001Sequence:
ATTCAGGCTGCGCAACTGTTSize:
20
Probe
Name:
TM031Sequence:
CGGTCCTCCGATCGCCCTTCCSize:
21
-
Amplicon:
Sequence:
gcaaaaaagcggttagctcctncggtcctccgatcgcccttccnaacagttgcgcagcctgaatSize:
64
Documents
Citation | Type | Local copy |
---|---|---|
EU-RL GMFF GMOMETHODS database; QT-EVE-GM-004 | EU reference method protocol |