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Detection Image Detection method details

Name:

 QL-ELE-T-g7-F/T-g7-R

Description:

 Qualitative real-time PCR (TaqMan) method for detection of the g7 terminator from Agrobacterium tumefaciens (Debode et al., 2013)

Comment:

 Target is the Agrobacterium tumefaciens g7 terminator (T-g7)

T-g7 (aka T-transcript7 or T-tr7) also recognises ORF23 of Agrobacterium tumefaciens since this is encoded by the reverse strand of T-g7.

Validation:

 in-house validation

Standardisation:

 none

Related Methods:

Type:

 element-specific

Target DNA element:
T-transcript 7, T-orf23-RHIRD

  • Oligonucleotides:

  • Forward Primer

  • Name:

    T-g7-F

  • Sequence:

    ATGCAAGTTTAAATTCAGAAATATTTCAA

  • Size:

    29

  • Reverse Primer

  • Name:

    T-g7-R

  • Sequence:

    ATGTATTACACATAATATCGCACTCAGTCT

  • Size:

    30

  • Probe

  • Name:

    T-g7-P

  • Sequence:

    ACTGATTATATCAGCTGGTACATTGCCGTAGATGA

  • Size:

    35

  • Amplicon:

  • Sequence:

    -

  • Size:

    97

Documents

Citation Type Local copy