Detection method details
Name: |
QL-ELE-bar 2-5'/bar 2-3' |
Description: |
Qualitative real-time PCR (TaqMan) method for detection of the bar gene (Mano et al., 2009) |
Comment: |
target is the phosphinothricin N-acetyltransferase (bar) gene from bacterium Streptomyces hygroscopicus |
Validation: |
in-house validation |
Standardisation: |
none |
Related Methods: |
Type: |
element-specific |
Target DNA element: |
CS-bar-STRHY
|
-
Oligonucleotides:
Forward Primer
Name:
bar 2-5'Sequence:
actgggctccacgctctacaSize:
20
Reverse Primer
Name:
bar 2-3'Sequence:
aaacccacgtcatgccagttcSize:
21
Probe
Name:
Bar-1-TaqSequence:
atgctgcgggcggccggcttcaagcacggSize:
29
-
Amplicon:
Sequence:
actgggctccacgctctacacccacctgctgaagtccctggaggcacagggcttcaagagcgtggtcgctgtcatcgggctgcccaacgacccgagcgtgcgcatgcacgaggcgctcggatatgccccccgcggcatgctgcgggcggccggcttcaagcacgggaactggcatgacgtgggtttSize:
186