Detection method details
Name: |
QL-ELE-cry3A-F/cry3A-R |
Description: |
Qualitative real-time PCR (TaqMan) method for detection of the cry3A, the modified mcry3A, the cry3A083, the cry3A085 and the chimaera cry3A/cry1Ab gene (Prins et al., 2016) |
Comment: |
target is the cry3A gene from bacterium Bacillus thuringiensis subsp. kumamotoensis (amplicon sequence taken from GenBank DI157749.1; MIR604 maize). |
Validation: |
in-house validation |
Standardisation: |
national standard |
Related Methods: |
Type: |
element-specific |
Target DNA element: |
CS-mcry3A-SYNTH, CS-cry3A-BACTT
|
-
Oligonucleotides:
Forward Primer
Name:
cry3A-FSequence:
cagcaacatcgagaactaSize:
18
Reverse Primer
Name:
cry3A-RSequence:
ctccagtagttgaagctgSize:
18
Probe
Name:
cry3A-PSequence:
tgttcgactacctgcaccgcSize:
20
-
Amplicon:
Sequence:
cagcaacatcgagaactacatccgcaagccccacctgttcgactacctgcaccgcatccagttccacacgcgtttccagcccggctactacggcaacgacagcttcaactactggagSize:
117