Detection method details
Name: |
QT-EVE-BN-004 |
Description: |
Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event GT73 (EU-RL GMFF GMOMETHODS database as of October 01, 2014) |
Comment: |
Target is the 3' integration border region (IBR) between the insert of oilseed rape event GT73 and the oilseed rape host genome |
Validation: |
ring trial validation |
Standardisation: |
EU reference method |
Related Methods: |
QT-TAX-BN-012, QT-TAX-FatA primer1/FatA primer2 |
Type: |
event-specific |
Target GMO name: |
GT73 |
-
Oligonucleotides:
Forward Primer
Name:
RT73 primer 1Sequence:
CCATATTGACCATCATACTCATTGCTSize:
26
Reverse Primer
Name:
RT73 primer 2Sequence:
GCTTATACGAAGGCAAGAAAAGGASize:
24
Probe
Name:
RT73 ProbeSequence:
TTCCCGGACATGAAGATCATCCTCCTTSize:
27
-
Amplicon:
Sequence:
ccatattgaccatcatactcattgctnnnnnnnnnnnnnttcccggacatgaagatcatcctccttnnnnnnnnnnnnnnnnnntccttttcttgccttcgtataagcSize:
108
Documents
Citation | Type | Local copy |
---|---|---|
EU-RL GMFF GMOMETHODS database; QT-EVE-BN-004 | EU reference method protocol |