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Detection Image Detection method details

Name:

 QT-TAX-SPS primer f/SPS primer r

Description:

 Quantitative real-time PCR (TaqMan) method for detection of the rice sucrose phosphate synthase (SPS) gene (ISO21570 Amd1)

Comment:

 Target is the endogenous sucrose phosphate synthase (SPS) gene from Oryza sativa.

BLAST analysis against GenBank (February 2024) shows two mismatches between the sequence of the forward primer and 12 of the 14 listed rice sequences (including the rice reference genome Nipponbare). First analyses performed by WFSR indicated that an adaptation of the forward primer sequence leads to an increased sensitivity in the detection of several rice varieties.

Validation:

 ring trial validation

Standardisation:

 ISO standard

Related Methods:

Type:

 taxon-specific

Target Species name:

 Oryza sativa

  • Oligonucleotides:

  • Forward Primer

  • Name:

    SPS primer f

  • Sequence:

    TTGCGCCTGAACGGATAT

  • Size:

    18

  • Reverse Primer

  • Name:

    SPS primer r

  • Sequence:

    CGGTTGATCTTTTCGGGATG

  • Size:

    20

  • Probe

  • Name:

    SPS probe

  • Sequence:

    TCCGAGCCGTCCGTGCGTC

  • Size:

    19

  • Amplicon:

  • Sequence:

    ttgcgcctgaacggatatctttcagtttgtaaccaccggatgacgcacggacggctcggatcatcccgaaaagatcaaccg

  • Size:

    81

Documents

Citation Type Local copy
Yang L., Yang Y., Jin W., Zhang X., Li X., Wu Y., Li J., Li L. (2018) Development and interlaboratories validation of event-specific quantitative real-time PCR method for genetically modified rice G6H1 event. Journal of Agricultural and Food Chemistry, 66(30),8179-8186Link to the document url. peer-reviewed article
Ding J, Jia, J, Yang L, Wen H, Zhang C, Liu W, Zhang D (2004) Validation of a rice specific gene, sucrose phosphate synthase, used as the endogenous reference gene for qualitative and real-time quantitative PCR detection of transgenes. J Agric Food Chem 5Link to the document url.