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Detection Image Detection method details

Name:

 QL-ELE-00-007

Description:

 Qualitative conventional PCR method for detection of nopaline synthase terminator (EU-Project SMT4-CT96-2072:1998)

Comment:

 Target is the nopaline synthase terminator (T-nos) from Agrobacterium tumefaciens

Validation:

 ring trial validation

Standardisation:

 national standard

Related Methods:

Type:

 element-specific

Target DNA element:
T-nos-RHIRD

  • Oligonucleotides:

  • Forward Primer

  • Name:

    NOS-1

  • Sequence:

    GAATCCTGTTGCCGGTCTTG

  • Size:

    20

  • Reverse Primer

  • Name:

    NOS-3

  • Sequence:

    TTATCCTAGTTTGCGCGCTA

  • Size:

    20

  • Amplicon:

  • Sequence:

    gaatcctgttgccggtcttgnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntagcgcgcaaactaggataa

  • Size:

    180

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QL-ELE-00-007Link to the document url. EU reference method protocol