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Detection Image Detection method details

Name:

 QT-TAX-ZM-004

Description:

 Quantitative real-time PCR (TaqMan) for detection of maize aldolase 1 (ald1) gene (EU-RL GMFF GMOMETHODS database as of July 14, 2016).

Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2

Comment:

 Target the aldolase 1 (ald1) gene from Zea mays.

This assay is NOT recommended for GMO quantitative analysis because of a secondary target with mismatches in the primers and probe annealing region.

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

Type:

 taxon-specific

Target Species name:

 Zea mays

  • Oligonucleotides:

  • Forward Primer

  • Name:

    Aldolase primer F

  • Sequence:

    AGGGAGGACGCCTCCCT

  • Size:

    17

  • Reverse Primer

  • Name:

    Aldolase primer R

  • Sequence:

    ACCCTGTACCAGAAGACCAAGG

  • Size:

    22

  • Probe

  • Name:

    Aldolase probe

  • Sequence:

    TGAGGACATCAACAAAAGGCTTGCCA

  • Size:

    26

  • Amplicon:

  • Sequence:

    agggaggacgcctccctccttgaggacatcaacaaaaggcttgccatccttggtcttctggtacagggt

  • Size:

    69

Documents

Citation Type Local copy
Jacchia S., Kagkli D.F., Lievens A., Angers-Loustau A., Savini C., Emons H., Mazzara M. (2018) Identification of single target taxon-specific reference assays for the most commonly genetically transformed crops using digital droplet PCR. Food Control 93,191-200Link to the document url. peer-reviewed article
EU-RL GMFF GMOMETHODS database; QT-TAX-ZM-004Link to the document url. EU reference method protocol