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Detection Image Detection method details

Name:

 QT-EVE-BN-012

Description:

 Quantitative real-time PCR (TaqMan) method for detection of oilseed rape event LBFLFK (EU-RL GMFF GMOMETHODS database as of October 12, 2022)

Comment:

 Target is the 3' integration border region (IBR) between the insert of oilseed rape event LBFLFK locus1 and the oilseed rape host genome.

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

 QT-TAX-BN-001

Type:

 event-specific

Target GMO name:

 LBFLFK

  • Oligonucleotides:

  • Forward Primer

  • Name:

    leftFST_ev1_loc1-f4

  • Sequence:

    ACCATCATACTCATTGCTGATCC

  • Size:

    23

  • Reverse Primer

  • Name:

    leftFST_ev1_loc1-r5

  • Sequence:

    TTCCTGTATACGCACACATAGTATA

  • Size:

    25

  • Probe

  • Name:

    leftFST_ev1_loc1-s2

  • Sequence:

    ATGTAGATTTCCCGGACATGAAGCCA

  • Size:

    26

  • Amplicon:

  • Sequence:

    accatcatac tcattgctga tccatgtaga tttcccggac atgaagccan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntatacta tgtgtgcgta tacaggaa

  • Size:

    128

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-EVE-BN-012Link to the document url. EU reference method protocol