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Detection Image Detection method details

Name:

 QL-TAX-SL-005

Description:

 Qualitative conventional PCR method for detection of tomato LAT52 gene (Yang et al., 2008).

Comment:

 target is the endogenous LAT52 gene from tomato

Validation:

 ring trial validation

Standardisation:

 unknown

Related Methods:

Type:

 taxon-specific

Target Species name:

 Solanum lycopersicum

  • Oligonucleotides:

  • Forward Primer

  • Name:

    Lat1

  • Sequence:

    AGACCACGAGAACGATATTTGC

  • Size:

    22

  • Reverse Primer

  • Name:

    Lat2

  • Sequence:

    TTCTTGCCTTTTCATATCCAGACA

  • Size:

    24

  • Amplicon:

  • Sequence:

    agaccacgagaacgatatttgcnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntgtctggatatgaaaaggcaagaa

  • Size:

    92

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QL-TAX-SL-005Link to the document url. EU reference method protocol
Yang L., Zhang H., Guo J., Pan L., Zhang D.; International Collaborative Study of the Endogenous Reference Gene LAT52 Used for Qualitative and Quantitative Analyses of Genetically Modified Tomato J. Agric. Food Chem. 56:3438-3443 (2008)Link to the document url. peer-reviewed article