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Detection Image Detection method details

Name:

 QL-ELE-00-013

Description:

 Qualitative real-time PCR (TaqMan) method for detection of nopaline synthase terminator (partim T-nos).

Part of the qualitative duplex real-time PCR (TaqMan) method for detection of Cauliflower mosaic virus 35S promoter and nopaline synthase terminator (partim T-nos) (ISO 21569 AMD1)

Comment:

 Target is the nopaline synthase terminator (T-nos) from Agrobacterium tumefaciens

For details of the detection of CaMV 35S promoter by this duplex real-time PCR method refer to QL-ELE-00-012

Validation:

 ring trial validation

Standardisation:

 ISO standard

Related Methods:

Type:

 element-specific

Target DNA element:
T-nos-RHIRD

  • Oligonucleotides:

  • Forward Primer

  • Name:

    180-F

  • Sequence:

    CATGTAATGCATGACGTTATTTATG

  • Size:

    25

  • Reverse Primer

  • Name:

    180-R

  • Sequence:

    TTGTTTTCTATCGCGTATTAAATGT

  • Size:

    25

  • Probe

  • Name:

    TM-180YY

  • Sequence:

    ATGGGTTTTTATGATTAGAGTCCCGCAA

  • Size:

    28

  • Amplicon:

  • Sequence:

    catgtaatgcatgacgttatttatgnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnacatttaatacgcgatagaaaacaa

  • Size:

    84

Documents

Citation Type Local copy
Waiblinger H.-U., Ernst B., Anderson A., Pietsch K.; Validation and collaborative study of a P35S and T-nos duplex real-time PCR screening method to detect genetically modified organisms in food products Eur. Food Res. Technol. 226:1221-1228 (2008)Link to the document url. peer-reviewed article
EU-RL GMFF GMOMETHODS database; QL-ELE-00-013Link to the document url. EU reference method protocol