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Detection Image Detection method details

Name:

 QT-TAX-BN-012

Description:

 Quantitative real-time PCR (TaqMan) method for detection of oilseed rape cruciferin A (CruA) gene (EU-RL GMFF GMOMETHODS database as of March 06, 2014).

Estimated number of target copies per haploid genome (Jacchia et al. 2018): 2

Comment:

 Target is the endogenous cruciferin A (CruA) gene from Brassica napus. Target range extends to B. juncea, B. rapa and B. oleracea.

Please be aware that additionally a cross-reactivity/positive signal with mustard (Sinapis alba) has been noticed.

This assay is NOT recommended for GMO quantitative analysis because of a secondary target with mismatches in the primers and probe annealing region.

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

Type:

 taxon-specific

Target Species name:

 Brassica napus

  • Oligonucleotides:

  • Forward Primer

  • Name:

    MDB510

  • Sequence:

    GGCCAGGGTTTCCGTGAT

  • Size:

    18

  • Reverse Primer

  • Name:

    MDB511

  • Sequence:

    CCGTCGTTGTAGAACCATTGG

  • Size:

    21

  • Probe

  • Name:

    TM003

  • Sequence:

    AGTCCTTATGTGCTCCACTTTCTGGTGCA

  • Size:

    29

  • Amplicon:

  • Sequence:

    ggccagggtttccgtgatntgcaccagaaagtggagcacataaggactnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnccaatggttctacaacgacgg

  • Size:

    101

Documents

Citation Type Local copy
Jacchia S., Kagkli D.F., Lievens A., Angers-Loustau A., Savini C., Emons H., Mazzara M. (2018) Identification of single target taxon-specific reference assays for the most commonly genetically transformed crops using digital droplet PCR. Food Control 93,191-200Link to the document url. peer-reviewed article
EU-RL GMFF GMOMETHODS database; QT-TAX-BN-012Link to the document url. EU reference method protocol