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Detection Image Detection method details

Name:

 QT-ELE-P35S 1-5'/P35S 1-3'

Description:

 Quantitative real-time PCR (TaqMan) method for detection of the Cauliflower mosaic virus 35S promoter (Kodama et al., 2009)

Comment:

 target is the 35S promoter (P-35S) sequence from the Cauliflower mosaic virus (CaMV)

Validation:

 ring trial validation

Standardisation:

 national standard

Related Methods:

Type:

 element-specific

Target DNA element:
P-Cauliflower mosaic virus

  • Oligonucleotides:

  • Forward Primer

  • Name:

    P35S 1-5'

  • Sequence:

    ATTGATGTGATATCTCCACTGACGT

  • Size:

    25

  • Reverse Primer

  • Name:

    P35S 1-3'

  • Sequence:

    CCTCTCCAAATGAAATGAACTTCCT

  • Size:

    25

  • Probe

  • Name:

    P35S-Taq

  • Sequence:

    CCCACTATCCTTCGCAAGACCCTTCCT

  • Size:

    27

  • Amplicon:

  • Sequence:

    CCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCACATCAAT

  • Size:

    100

Documents

Citation Type Local copy