Help icon

Detection Image Detection method details

Name:

 QL-CON-00-012

Description:

 Qualitative real-time PCR (TaqMan) method for detection of papaya event 55-1 and 63-1 (ISO 21569)

Comment:

 Target is the junction region between the cauliflower mosaic virus 35S promoter (P-35S CaMV) and the chimeric cucumber mosaic virus coat protein/papaya ring spot virus coat protein

Validation:

 ring trial validation

Standardisation:

 ISO standard

Related Methods:

Type:

 construct-specific

Target GMO names:
55-1, 63-1

  • Oligonucleotides:

  • Forward Primer

  • Name:

    35S-F

  • Sequence:

    GACGTAAGGGATGACGCACAA

  • Size:

    21

  • Reverse Primer

  • Name:

    sunup-ar1

  • Sequence:

    ACGTCGTCAGTCCAAGAATGA

  • Size:

    21

  • Probe

  • Name:

    35S -T

  • Sequence:

    CCCACTATCCTTCGCAAGACCCTTCC

  • Size:

    26

  • Amplicon:

  • Sequence:

    gacgtaagggatgacgcacaancccactatccttcgcaagacccttccnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnacgtcgtcagtccaagaatga

  • Size:

    220

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QL-CON-00-012Link to the document url. EU reference method protocol