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Detection Image Detection method details

Name:

 QT-EVE-ZM-033

Description:

 Quantitative real-time PCR (TaqMan) method for detection of maize event MON95379 (EU-RL GMFF GMOMETHODS database as of December 20, 2022)

Comment:

 Target is the 3' integration border region (IBR) between the insert of maize event MON 95379 and the maize host genome

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

 QT-TAX-ZM-002

Type:

 event-specific

Target GMO name:

 MON95379

  • Oligonucleotides:

  • Forward Primer

  • Name:

    MON 95379 primer 1

  • Sequence:

    CCAAGAAGAACGATTGGCAAAC

  • Size:

    22

  • Reverse Primer

  • Name:

    MON 95379 primer 2

  • Sequence:

    GGCACAGGCACGCCTCTG

  • Size:

    18

  • Probe

  • Name:

    MON 95379 probe

  • Sequence:

    ATGGGTATTATGGGTAGGCACATGGGAATATAG

  • Size:

    33

  • Amplicon:

  • Sequence:

    ccaagaagaa cgattggcaa acnnnnnnna tgggtattat gggtaggcac atgggaatat agnnnnnnnc agaggcgtgc ctgtgcc

  • Size:

    87

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-EVE-ZM-033Link to the document url. EU reference method protocol