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Detection Image Detection method details

Name:

 QT-EVE-ZM-030

Description:

 Quantitative PCR method for detection of maize event MON87429 (EU-RL GMFF GMOMETHODS database as of August 30, 2021)

Comment:

 Target is the 5' integration border region (IBR) between the insert of maize event MON87429 and the maize host genome

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

 QT-TAX-ZM-002

Type:

 event-specific

Target GMO name:

 MON87429

  • Oligonucleotides:

  • Forward Primer

  • Name:

    MON 87429 primer 1

  • Sequence:

    CGAGACAGACTCAATGTATCCGAGATACTC

  • Size:

    30

  • Reverse Primer

  • Name:

    MON 87429 primer 2

  • Sequence:

    CCATCATACTCATTGCTGATCCATGTA

  • Size:

    27

  • Probe

  • Name:

    MON 87429 probe

  • Sequence:

    TCCCGGACATGAAACCAAACAAGAGTGGTC

  • Size:

    30

  • Amplicon:

  • Sequence:

    cgagacagac tcaatgtatc cgagatactc nnnnnnnnnn nnnnnnnnnn nnnnngacca ctcttgtttg gtttcatgtc cgggannnnt acatggatca gcaatgagta tgatgg

  • Size:

    116

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-EVE-ZM-030Link to the document url. EU reference method protocol