Detection method details
Name: |
QL-CON-00-005 |
Description: |
Qualitative conventional PCR method for detection of maize event T25 (ISO 21569) |
Comment: |
Target is the junction region between the phosphinothricin N-acetyltransferase (CS-pat-STRVR) gene from Streptomyces viridochromogenes and the Cauliflower mosaic virus 35S terminator (T-35S-CaMV) |
Validation: |
ring trial validation |
Standardisation: |
ISO standard |
Related Methods: |
QL-TAX-ZM-003 |
Type: |
construct-specific |
Target GMO names: |
32316, 40416, 4114, 43A47, 676, 678, 680, A2704-12, A2704-21, A5547-127, A5547-35, DAS1507, DAS59122, DAS59132, DBN9004, DP62151, Falcon GS 40/90, GU262, HCN10, HCR-1, Liberator, T120-7, T14, T25, T45, Topas 19/2
|
-
Oligonucleotides:
Forward Primer
Name:
T25-F7Sequence:
ATGGTGGATGGCATGATGTTGSize:
21
Reverse Primer
Name:
T25-R3Sequence:
TGAGCGAAACCCTATAAGAACCCSize:
23
-
Amplicon:
Sequence:
atggtggatggcatgatgttgnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnngggttcttatagggtttcgctcaSize:
209
Documents
| Citation | Type | Local copy |
|---|---|---|
EU-RL GMFF GMOMETHODS database; QL-CON-00-005 |
EU reference method protocol | |
| Foodstuffs - Methods of analysis for the detection of genetically modified organisms and derived products - Qualitative nucleic acid based methods ISO 21569:1-69 (2005) |
other document |