Detection method details
Name: |
QL-EVE-558F/558R |
Description: |
Qualitative real-time PCR (TaqMan) method for detection of Bacillus subtilis isolate e871 (Paracchini et al., 2017). |
Comment: |
Target is the integration site between the inserted cat gene and the endogenous recA gene of Bacillus subtilis. |
Validation: |
unknown |
Standardisation: |
none |
Related Methods: |
Type: |
event-specific |
Target GMO name: |
e871 Bacillus subtilis |
-
Oligonucleotides:
Forward Primer
Name:
558-FSequence:
CGAGCTTTTGCGCGTATASize:
18
Reverse Primer
Name:
558-RSequence:
GCCATTCCAATACAAAACCACATASize:
24
Probe
Name:
558-TmSequence:
CGGATCTAACGCATGCTCCGCASize:
22
-
Amplicon:
Sequence:
cgagcttttgcgcgtataccggatctaacgcatgctccgcatcgattataggtatgtggttttgtattggaatggcSize:
76
Documents
Citation | Type | Local copy |
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