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Detection Image Detection method details

Name:

 QT-TAX-OS-002

Description:

 Quantitative real-time PCR (TaqMan) method for detection of rice phospholipase D alpha 2 gene (EU-RL GMFF GMOMETHODS database as of April 19, 2016)

Comment:

 Target is the phospholipase D alpha 2 (PLD) gene from rice.

Amplicon sequence based on GenBank AP014962 (Oryza sativa Japonica, cultivar: Nipponbare) and GenBank CP012614.1 (Oryza sativa Indica cultivar: RP Bio-226)

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

Type:

 taxon-specific

Target Species name:

 Oryza sativa

  • Oligonucleotides:

  • Forward Primer

  • Name:

    PLD-GR For

  • Sequence:

    GGCAAGAACAACACCATTGACA

  • Size:

    22

  • Reverse Primer

  • Name:

    PLD-GR Rev

  • Sequence:

    CGGCTTTCCATGCAAATGA

  • Size:

    19

  • Probe

  • Name:

    PLD-GR Probe

  • Sequence:

    ATTCGCCGCGCCAAGAACTTCA

  • Size:

    22

  • Amplicon:

  • Sequence:

    GGCAAGAACAACACCATTGACAGGAGCATCCAAGATGCATACATCCACGCAATTCGCCGCGCCAAGAACTTCATCTACATCGAGAATCAGTACTTCCTTGGCAGCTCATTTGCATGGAAAGCCG

  • Size:

    124

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-TAX-OS-002Link to the document url. EU reference method protocol