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Detection Image Detection method details

Name:

 QT-EVE-ZM-010

Description:

 Quantitative real-time PCR (TaqMan) method for detection of maize event DAS1507 (EU-RL GMFF GMOMETHODS database as of March 17, 2014)

Comment:

 Target is the integration border region (IBR) between the insert of maize event DAS1507 and the maize host genome

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

 QT-TAX-ZM-002

Type:

 event-specific

Target GMO name:

 DAS1507

  • Oligonucleotides:

  • Forward Primer

  • Name:

    MaiY-F1

  • Sequence:

    TAGTCTTCGGCCAGAATGG

  • Size:

    19

  • Reverse Primer

  • Name:

    MaiY-R3

  • Sequence:

    CTTTGCCAAGATCAAGCG

  • Size:

    18

  • Probe

  • Name:

    MaiY-S1

  • Sequence:

    TAACTCAAGGCCCTCACTCCG

  • Size:

    21

  • Amplicon:

  • Sequence:

    tagtcttcggccagaatggcctaactcaaggccctcactccgcttgatcttggcaaag

  • Size:

    58

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-EVE-ZM-010Link to the document url. EU reference method protocol