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Detection Image Detection method details

Name:

 QT-EVE-ZM-003

Description:

 Quantitative PCR method for detection of maize event MON87427 (EU-RL GMFF GMOMETHODS database as of April 21, 2017).

Comment:

 Target is the 5' integration border region (IBR) between the insert of maize event MON87427 and the maize host genome.

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

 QT-TAX-ZM-002

Type:

 event-specific

Target GMO name:

 MON87427

  • Oligonucleotides:

  • Forward Primer

  • Name:

    MON87427 primer1

  • Sequence:

    ACGGAAACGGTCGGGTCAAATG

  • Size:

    22

  • Reverse Primer

  • Name:

    MON87427 primer 2

  • Sequence:

    CCATGTAGATTTCCCGGTTTTCTC

  • Size:

    24

  • Probe

  • Name:

    MON87427 probe

  • Sequence:

    TCGGGACAATATGGAGAAAAAGAAAGAG

  • Size:

    28

  • Amplicon:

  • Sequence:

    acggaaacgg tcgggtcaaa tgnnnnnnnt cgggacaata tggagaaaaa gaaagagnnn nnnnnnnnnn ngagaaaacc gggaaatcta catgg

  • Size:

    95

Documents

Citation Type Local copy
EU-RL GMFF GMOMETHODS database; QT-EVE-ZM-003Link to the document url. EU reference method protocol