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Detection Image Detection method details

Name:

 QT-EVE-GH-006

Description:

 Quantitative real-time PCR (TaqMan) method for detection of cotton event GHB614 (EU-RL GMFF GMOMETHODS database as of January 13, 2014)

Comment:

 Target is the 3' integration border region (IBR) between the insert of cotton event GHB614 and the cotton host genome

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

 QT-TAX-GH-019

Type:

 event-specific

Target GMO name:

 GHB614

  • Oligonucleotides:

  • Forward Primer

  • Name:

    SHA007

  • Sequence:

    CAAATACACTTGGAACGACTTCGT

  • Size:

    24

  • Reverse Primer

  • Name:

    SHA008

  • Sequence:

    GCAGGCATGCAAGCTTTTAAA

  • Size:

    21

  • Probe

  • Name:

    TM072

  • Sequence:

    CTCCATGGCGATCGCTACGTATCTAGAATT

  • Size:

    30

  • Amplicon:

  • Sequence:

    caaatacacttggaacgacttcgtnnnnnnctccatggcgatcgctacgtatctagaattnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnntttaaaagcttgcatgcctgc

  • Size:

    120

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Citation Type Local copy