Help icon

Detection Image Detection method details

Name:

 QL-EVE-816AvaF/817AvaR

Description:

 Qualitative real-time PCR (TaqMan) method for detection of oilseed rape event Falcon GS40/90 (integration locus 1 – Avalon) (BVL G30.40-6).

Comment:

 Target is the integration border region (IBR) between the insert of oilseed rape event Falcon GS40/90 and the rapeseed host genome (integration locus 1 – Avalon).

Validation:

 ring trial validation

Standardisation:

 national standard

Related Methods:

 QT-TAX-BN-012

Type:

 event-specific

Target GMO name:

 Falcon GS 40/90

  • Oligonucleotides:

  • Forward Primer

  • Name:

    816AvaF

  • Sequence:

    GAAAACGACAAAAACTGCGAAAT

  • Size:

    23

  • Reverse Primer

  • Name:

    817AvaR

  • Sequence:

    TGGCTTGTGTTTTATGTTTGGAAA

  • Size:

    24

  • Probe

  • Name:

    818AvaS

  • Sequence:

    TGCGGGCAGTGCCTTCAGTTTTCC

  • Size:

    24

  • Amplicon:

  • Sequence:

    gaaaacgacaaaaactgcgaaatttgcgggcagtgccttcagttttcctattaatatttagtttgacaccagttgcaaatataacattataaaccacgtgcatggtctcatgtgaacacactcattttaaatattttcgacgtttccaaacataaaacacaagcca

  • Size:

    166

Documents

Citation Type Local copy
Real-time PCR detection of genetically modified oilseed rape lines Falcon GS40/90 and Liberator pHoe6/Ac - event-specific methods. BVL Official Method Collection G 30.40-06 Online Publication (2013)Link to the document url. other document