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Detection Image Detection method details

Name:

 QL-CON-00-013

Description:

 Qualitative real-time PCR (TaqMan) method for detection of the junction between the maize ubiquitin promoter and the modified cry1Ab/1Ac genes (Grohmann et al.,2015).

Comment:

 Target is the junction region between the ubiquitin promoter (P-ubi) sequence from maize (Zea mays) and the cry1Ab/Ac genes from Bacillus thuringiensis.

Validation:

 ring trial validation

Standardisation:

 EU reference method

Related Methods:

Type:

 construct-specific

Target GMO names:
3006-210-23, 4114, DAS1507, DAS59122, DAS59132, KMD1, Kefeng6

  • Oligonucleotides:

  • Forward Primer

  • Name:

    pubi-F2

  • Sequence:

    ATTTGCTTGGTACTGTTTCTTTTGTC

  • Size:

    26

  • Reverse Primer

  • Name:

    cry-rr-R

  • Sequence:

    TTGTTGTCCATGGATCCTCTAGAGT

  • Size:

    25

  • Probe

  • Name:

    pubi-T2

  • Sequence:

    ACCCTGTTGTTTGGTGTTACTTCTGCA

  • Size:

    27

  • Amplicon:

  • Sequence:

    atttgcttggtactgtttcttttgtcgatgctcaccctgttgtttggtgttacttctgcaggtcgactctagaggatccatggacaacaa

  • Size:

    90

Documents

Citation Type Local copy
Grohmann L., Reiting R., Maede D., Uhlig S., Simon K., Frost K., Jit Randhawa G., Zur K. Collaborative trial validation of cry1Ab/Ac and Pubi-cry TaqMan-based real-time PCR assays for detection of DNA derived from genetically modified Bt plant products. Accred Qual Assur 20:85-96 (2015)Link to the document url. peer-reviewed article
EU-RL GMFF GMOMETHODS database; QL-CON-00-013Link to the document url. EU reference method protocol