Detection method details
Name: |
QL-ELE-00-003 |
Description: |
Qualitative conventional PCR method for detection of the neomycin phosphotransferase II gene (nptII) (BVL L 00.00-31, 2001) |
Comment: |
target is the Neomycin phosphotransferase II (nptII) gene |
Validation: |
ring trial validation |
Standardisation: |
national standard |
Related Methods: |
Type: |
element-specific |
Target DNA element: |
CS-nptII-ECOLX, V-nptII-ECOLX
|
-
Oligonucleotides:
Forward Primer
Name:
TN5-1Sequence:
GGATCTCCTGTCATCTSize:
16
Reverse Primer
Name:
TN5-2Sequence:
GATCATCCTGATCGACSize:
16
-
Amplicon:
Sequence:
ggatctcctgtcatctnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnngtcgatcaggatgatcSize:
173
Documents
| Citation | Type | Local copy |
|---|---|---|